Environment is intended for the isolation and cultivation of the infected material Listeria when added selective agents.
Nutrient agar dry fodder yeast extract, dextrose, thiamin bromide, disodium phosphate.
43.5 grams of the drug mix in 1 liter of distilled water, bring to a boil, boil for 2 minutes. Before sterilization make nalidixic acid (0.04 g nalidiks.kisloty dissolved in 0.1 ml of 1% NaOH by adding 1.5 ml of distilled water) and add to 1 liter of medium. Autoclaving at a temperature of 115º C for 30 min. Before use, sterile environment cooled to a temperature (47,5+ 2,5) º C and pour into sterile Petri dishes.