Nutrient medium Olkenitsky

Specification

A breeding ground for identification of Enterobacteriaceae Dry (Iron-glucose-lactose agar)

Reg. VFS number 42-416 BC-93

Feature:

Wednesday is intended for generic identification of enterobacteria by tests: utilization of urea, hydrogen sulfide, fermentation of carbohydrate - lactose, glucose.

Ingredients:

Pancreatic hydrolyzate of fish meal with sodium thiosulfate, lactose, glucose, sodium phosphate dibasic, potassium dihydrogen phosphate, sodium chloride, iron oxide citrate, phenol red, sodium sulfite, urea agar.

Preparation:

Drug in an amount necessary for the preparation of the series of culture medium again stirred in 1 liter of distilled water and sterilized by autoclaving at 110 ° C for 15 min. After sterilization, the cooled medium to a temperature 40-50oS poured into 7 ml sterile tubes and Mow, leaving a column height of 25-30 mm.

Prepared medium is red.

Sterile medium can be used for two weeks when it is stored at a temperature or 2-8oS for 3 days at a storage temperature 18-25oS (vials should be stored in a dark place).

pH 7,1 ± 0,2.