(Nutrient medium for the isolation and cultivation of Vibrio cholerae dry)
The nutrient medium is used for the cultivation of Vibrio cholerae and isolating it from the infected material.
Is a fine powder from light yellow to light brown, hygroscopic.
Pancreatic hydrolyzate of fish meal, peptone, dry fermentation, yeast extract, glucose, sodium phosphate dibasic, sodium chloride, sodium metabisulfite, sodium carbonate agar.
Drug in an amount necessary for the preparation of a series of specific nutrient medium in a stirred 1 liter of distilled water, heated until complete melting of the agar, filtered through cotton-gauze filter, dispensed in vials and sterilized by autoclaving for 20 minutes at a temperature
(121 ± 1) ° C.
Ready nutrient medium transparent yellow. Light opalescence is permitted.
pH 8,0 ± 0,2
Prepared medium can be stored for 10-14 days at 2-8 ° C in a dark place.