Nutrient medium for the detection of toxigenic diphtheria Germs Dry (OTDM)
Reg. FS number 42-3519-98
Environment is intended to determine the microbial toxigenicity diphtheria precipitation method in agar.
Autolysate sprat, sodium phosphate dibasic, agar, soda ash.
A batch of the drug specified on the label, stir in 1 liter of distilled water, boil for 3-5 minutes until completely melted agar. Pour into sterile 17 ml tubes by aseptically and sterilized by autoclaving for 30 minutes at 110 ° C. The completed medium in the tubes can be stored at 6-8 ° C for 10 days. Before use, the medium in a water bath to melt and, after cooling to 40-50 ° C in a test tube add 3 ml of normal horse serum, previously tested for sterility. Stir the contents and pour into sterile Petri plates with a diameter of 10 ml.